Table 2A.

Specific lysis of Colo−/Colo+, CX−/CX+, and K562 target cells by differentially activated NK cells before and after blocking of the target cells with Hsp70 specific antibody cmHsp70.1

Target cells	Mean values of specific lysis (% ± SE)*
	Control	TKD	CX− exos	CX+ exos	CX− lys	CX+ lys
Colo−	8 ± 3	19 ± 2	11 ± 6	18 ± 3	nt	nt
Colo+	12 ± 4	28 ± 4†	13 ± 5	30 ± 4†	nt	nt
CX−	15 ± 5	43 ± 2.5	55 ± 4	50 ± 4	22 ± 6	20 ± 8
CX− plus cmHsp70.1	nt	43 ± 8	48 ± 9	41 ± 5	nt	nt
CX+	19 ± 4	78 ± 5†	62 ± 3	83 ± 3†	23 ± 2	22 ± 8
CX+ plus cmHsp70.1	nt	20 ± 4†	59 ± 4	43 ± 4†	nt	nt
K562	5 ± 1	63 ± 8†	20 ± 3.5	53 ± 7†	32 ± 2	32 ± 2
K562 plus cmHsp70.1	nt	19 ± 8†	25 ± 6	24 ± 8†	nt	nt

Abbreviation: lys, lysates; nt, not tested.

^*Lysis of Colo−/Colo+, CX−/CX+, and K562 target cells at a distinct E/T ratio of 10:1, by CD94+ and CD3⁻CD19⁻ NK cells stimulated either with 100 IU/mL IL-2 only (control) or with a combination consisting of low-dose IL-2 (100 IU/mL) plus TKD (2 μg/mL; TKD), plus CX− exosomes/CX+ exosomes, or plus CX− lysates/CX+ lysates, at a concentration of 10 μg/mL each. Data represent mean values of at least three independent experiments ± SE.

^†Means significantly different from the relevant counterpart (P < 0.005).