Table 2C.
Cell surface density and fold increase in cell surface markers on CD56+ NK cells stimulated either with Hsp70 peptide TKD (2 μg/mL), CX−/CX+ exosomes (10 μg/mL), and lysates (10 μg/mL) derived thereof as compared to unstimulated NK cells
NK cell marker | Mean fluorescence intensity (fold increase) |
||||
---|---|---|---|---|---|
TKD | CX− exos | CX+ exos | CX− lys | CX+ lys | |
CD94 | 336 (2.0)* | 227 (1.3) | 315 (1.9)* | 241 (1.4) | 241 (1.4) |
NKp30 | 108 (2.6)* | 104 (2.4)* | 117 (2.7)* | 101 (2.4)* | 103 (2.4)* |
NKp44 | 266 (2.2)* | 293 (2.4)* | 313 (2.6)* | 256 (2.1) | 293 (2.4)* |
NKp46 | 342 (1.3) | 320 (1.2) | 354 (1.4) | 347 (1.3) | 333 (1.3) |
NKG2D | 213 (1.1) | 276 (1.4) | 283 (1.4) | 273 (1.4) | 259 (1.3) |
CD69 | 185 (3.1)* | 139 (1.8) | 113 (2.4)* | 119 (1.8) | 118 (1.8) |
CD158a | 524 (1.2) | 560 (1.2) | 505 (1.1) | 433 (1.0) | 442 (1.0) |
NOTE: Data represent mean values of three independent experiments.
Means significantly different from unstimulated controls. Similar results were obtained if CD3/CD19-depleted NK cells were used. The percentage of CD3−/CD56+ NK cells in all experiments was always greater 85%.