Table 3. .
Primer Sequences for Sequencing Analysis
| Primer Sequencea(5′→3′) |
||
| Gene and Primer Name | Forward | Reverse |
| HFE2: | ||
| Ex2 | AGTCACTTACAGGGCTTCCG | TCACATGCCCACCCCTAC |
| Ex3_1 | AGTTATGAGCAAACTACACTCCG | GTCACAAGGGTCCGGGG |
| Ex3_2 | CGAAGACCTGATGATCCAGC | TGTTGAATAGTGGGGATGGG |
| Ex4_1 | TGTATGAGGTCTGATTGGGG | TTATAGCTCCCCGACGATTG |
| Ex4_2 | TCAAGGTAGCAGAGGATGTGG | GTCTCCTAGGCCCTGCTTC |
| LIX1L: | ||
| Ex1 | CTAGCTGACTGGGGTGGTTG | AAAAGCGATCCGGAGGAC |
| Ex2 | TAGGGAGCTAAGGGGCAGAC | TTTAATGGCCAAAGTGGTGG |
| Ex3 | CTGAAGTGTTTCCCTCCCAC | AATTTACGACTTCCCAAGCAG |
| Ex4 | actggacagtgctgCTTGAG | TCTTTTCCAGTATCAATGAGTTAGG |
| Ex5 | TCCATTTTGTAAAAGGTTTCCC | TTCACAACCATAGGTCACAATAGAC |
| Ex6 | CAGTTGGGTCAGTGTGGTATG | GGATCTCTTAGCAAATAGGAATCTAGG |
| PIAS3: | ||
| Ex1 | AGGGTGGAGAGTTGTGCG | ACCCAGTCTCGGTCGCTTC |
| Ex2_1 | ACTGCCCACCTGGTAGCTC | GGAATGGGAGCTAGAGGACC |
| Ex2_2 | CTTTCCCCGGAAGACCC | ATCCCAGGTCACTGATCCC |
| Ex3 | TATGGGATCAGTGACCTGGG | ggatagggaagagcacaggg |
| Ex4 | TTTCTGTGCATTCACCCTTG | AGCAGGGACATCTCGTAAGG |
| Ex5 | GGTTCCCTGTCTCTCTGCC | tgctaggcATGTCTCTTTCAG |
| Ex6 | CAGGGATTGAATAACCCGAG | CAACCCAGGACACTGGATG |
| Ex7 | CCTCATGGAACAAAAGTGGG | AATTATGAAGGCTGGAGGGC |
| Ex8 | CTGTTCCTGGCCTTACCAAC | CGCCAGTATCCCTAGGAACC |
| Ex9 | GGAAGGGAGTTGGGTTTAGC | ggggaacagagtaagattccatc |
| Ex10-11 | aaggtgagttacAGAACTCTGGC | TCCAATGATCAGAAAGCAATC |
| Ex12-13 | GGGACAGAGAGGGAACCTG | ATCATAAGAGAGGGAGGGGC |
| Ex14 | TTTCTGAGATGAGTGACTGGG | AGAGTAGCTGGGGTTGGGAC |
| ANKRD35: | ||
| Ex1 | CCAAGTCCGGTGGGTTC | ATGATGCATTGAGACGGGAC |
| Ex2 | TTGGCATCCTAACCATTTGC | GAGCTCTGAAGTTGTAGGCATTC |
| Ex3 | GGCTTATCAAGATTCTCCCATTC | AGTAGGAATGTCCAGCAGGG |
| Ex4 | CTGTGCGTCTCTTGCCTTC | ATGAGGCGAGTACATTTGGC |
| Ex5-6 | CCATGGAAATTGGTGACCC | GTCATGGAAGTTGGCCAGTC |
| Ex7 | GGGAGCCGGTAGGATTGTC | acacacacacacGTTGCCC |
| Ex8 | CCTGTGAGTGGAATGTCTGG | TCCATGGAAACAAATGGGAC |
| Ex9 | GAAGATAAGAATGTGGGCTGG | ACACCCCAAGGACCACTAGG |
| Ex10_1 | TGGGGTGTCAAGACATTTAAGAG | ATCTTTCCTGGGGCTGAGTC |
| Ex10_2 | CTAAGGACCTGCTGGCTGAG | CAGCACCCGCTCCAGTC |
| Ex10_3 | GGGGCTTTGTCAAGACCG | GCCAGTTCATTTGTCAGCAG |
| Ex10_4 | GGTTGCAGAGGGAGCTACAG | GCTTCAGCTTTCCGATCTCC |
| Ex10_5 | CTCTGGAGCAAGACCTGGG | ACCCACTGCTGCAATAAAGG |
| Ex11 | gaagctGACCTTGACCCTTG | AATTCCTAGAATGTCTCCCTCAG |
| Ex12 | GCTGACCACTTCATTGTGTTG | ACCCTGGAGAAGAGAGAGGG |
| Ex13 | CCACAGAACCTCTCCCTCAC | AACTCCTTCCCTCATCACCC |
| ITGA10: | ||
| Ex1 | GGGAGGGAAAGTGAAGAAAAC | CCAAAGAATTTTAACTATAATGGTCCC |
| Ex2 | ACGCTTCCTCACAGCTTCTC | TCCCATCTATCTTGCCTCCC |
| Ex3 | CTCCATGCTCTTTCCCTTTG | AAAAGCTGAGGTATAGGGAGTTG |
| Ex4 | CCCTGCTCATTCTCCTCC | GACCATTTTCCCTTACCACAC |
| Ex5 | TTCATGCCTCCTTCAACTGG | CCTTTGCCAGGCATCATAC |
| Ex6 | TCCTCCTGTGACCTCTTTCC | ctcaggggatcctcccac |
| Ex7 | TTCTTCTTCCTCCTCTCCCC | CCGCCCTAACTCTTCTAAGC |
| Ex8 | ATGGCCTAAAAGGCAAGATG | TCCCAGTCCTTGGACAGTTC |
| Ex9-10 | GACTTCAGCCACAGCATGTTAG | AAGGAGGAATCTGGAGGGC |
| Ex11 | CCCTCAGTGATCTTTTCACTCC | GAGTATTTCATACCCGCCCC |
| Ex12 | CTATGCCTTTAGGGAACCCC | ACTTCACACTCCTCCCCAAC |
| Ex13 | TCCCACTGAGTCCACTTGTC | CAACCTCTCAGCAAACCCTC |
| Ex14 | AGGAAAGGAGGGAAGGTGC | TTGACAAGCAAAGAGCATCC |
| Ex15 | aaaGAGCAAGGCAGGAGG | tgtgactttaaggcccttgc |
| Ex16-17 | AAACACTGGGCTTGCACTTC | AATTCCTCCTCTGACCCAGC |
| Ex18 | taggacagtgcccagcacac | TCTCCCTCCCTCCCTTTTC |
| Ex19-20 | TTTTGTCTTTTCACTTTCCCC | ACAGCTGAGGCCAATACCC |
| Ex21-22 | TCAGTGTTTAGAGGCTGTGGG | CTCCTCAGAAGACACCCTTCTC |
| Ex23 | GAGGGGTGGGGAGGAAC | GGGAGGGAAGCAGCATAAG |
| Ex24-25 | GAATCCACGTCTGTGTGCTG | TGGTGTCCAGCTTCAGTGAG |
| Ex26 | AAGGGGAAGAACTACAAGGG | CAGTTCCTACCCTCTTGTCCC |
| Ex27 | CTGTGTGAACTAGAGGTGGGG | acgtgggtctgtctacctcc |
| Ex28 | CCTCTCAGCCTCAACTTTGC | ACGGGTGGCCCTACCAC |
| Ex29 | GCTTTGATATAATGTGGGGTGAG | CCAGCACAAATCCTGTTCTC |
| Ex30 | CAGAACAAAAGTGAAGACCTCAG | CCCCAAACCTCTGCTTTTATG |
| RBM8A: | ||
| Ex1 | GCCTTTGATTGGTCAGCTTG | AGACCCGGTTCCTCGATTC |
| Ex2-3 | TGTATTGGGAAAGAGACGATTG | CTGAACCCATTCCTACTGCG |
| Ex4 | CGCAGTAGGAATGGGTTCAG | CCAACCACAGCAAACACAG |
| Ex5 | ACGCCAAAGAGACAGAAACG | AAAGAAGCAGGGAGAGGAGTC |
| Ex6 | ggtgaagggaatacgaacga | accctgggtaacacagcaag |
| PEX11B: | ||
| Ex1 | CGCAGTAGGCGTGACTAGG | GCCTAAACTGACTTCGCCTC |
| Ex2 | TCTGTCTGTGCCTTTTGGAAG | GGAAGGAGGGACAAAGGAAG |
| Ex3 | CTCAACTTTTGCCCTCTTGC | AAGTGATTGGAAGCCAAGTG |
| Ex4_1 | CTAATGTGTAATCTCTTTGCTACAGG | TCACAGGCATTTCTGACCAC |
| Ex4_2 | ACTCCAGGAGGAGGTCTGC | ATTCGAATGAGGCTGGCAC |
| POLR3GL: | ||
| Ex2 | TACCTCCTTGGCTTCCTGTC | ATGGTCCAGGAGGAATTCAG |
| Ex3 | ATTGGGAGGAGGGGTCTTC | GTATGCTTCCTGTGCCCCTC |
| Ex4 | GGCTGGACTCTCAGGAACAG | GCGTGGTGGGAATAGCTTG |
| Ex5-6 | TCCTTTAAAACCCTCACCCC | ACACATGGGCCAAGAGAG |
| Ex7 | TTCCCTCTCTCTTGGCCC | GGCATGCCAACCCTCTG |
| Ex8 | GGGGTCTGGTACACAGGAAG | TGATTTGCTTGTCTGAAGAAAC |
| TXNIP: | ||
| Ex1 | AAAGGGGTTTCCTCGATTTG | GCAACTTAAAACATTTCGTACAGG |
| Ex2-3 | CCACCCAAGCATTCCTTATC | GCTTCAATCTAATGCCCAAGAC |
| Ex4 | TCCTGGGTCATGAGGATG | TTCCTCTACCCCAGTCCC |
| Ex5 | GAACTGGGTTTAGGGGCAAG | GGTCTGGAGAAACAAGACAGC |
| Ex6 | TGGCTTCAGCAGAGAAATTG | GCCCAAGAAATGCTGGAC |
| Ex7-8 | CCAGAAGGTGAGCCAGACC | CTAAGGTGGACCCACACTCC |
| GNRR2: | ||
| Ex1_1 | gaaaaggagggcgaagaatc | ACGTGGCCATTAGTTTCAGG |
| Ex1_2 | AGCAGCCGCCGACTTACTA | agcagaggcttactccctga |
| Ex2-4 | ttgggaggagacagtatgaca | ccatggtttagtgactggtagat |
| Ex5_1 | ccctccatcaattcacccta | AGCATTCTCCCAGACCCTTC |
| Ex5_2 | CCCCACCATGCTAACTGAAG | GGGGAACTAGGAGAGCAGGA |
a
Lowercase letters represent intronic sequences.