Fig. 1.
DNA alcoholysis by BfiI. (A) Scheme for the hydrolysis and alcoholysis reactions of BfiI on the 14/15 duplex (for the nucleotide sequence, see Table 1). The star marks the 3′ radiolabel on the bottom strand. (B) Samples from reactions of BfiI on 14/15 in aqueous buffer (lane marked H2O) or in the presence of an alcohol at the concentration indicated above each lane were subjected to denaturing PAGE and analyzed by PhosphorImager. Lanes marked S and P contain the intact substrate and the chemically synthesized 5′-phosphorylated hydrolysis product of 15 and 13 nt, respectively.