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. 2007 Jan 31;104(7):2115–2120. doi: 10.1073/pnas.0608689104

Fig. 2.

Fig. 2.

DNA hairpin formation. (A) A scheme for the transesterification and hydrolysis reactions of BfiI on the 3′-tailed duplex, 25C/15 (Table 1) is shown. The star marks the 3′ radiolabel on the bottom strand. (B) Samples from reactions of BfiI on 25C/15 were subjected to denaturing PAGE and analyzed by PhosphorImager. Lanes marked M contain the intact 15-nt substrate (left) or chemically synthesized hydrolysis and hairpin products (right). (C) Quantitative data from the gel in B: open circles, intact substrate; filled circles, hydrolysis product; open diamonds, hairpin DNA.