Fig. 3.

Maxam–Gilbert sequencing. (A) A scheme for hairpin formation in the reaction of BfiI on 25C/15 is shown. The asterisk (∗) marks the ³³P label at the 5′ end of the top strand. (B) Maxam–Gilbert sequencing of the low-mobility product from the reaction of BfiI on 25C/15 and of the chemically synthesized hairpin DNA. The G, A+G, C+T, and A>C chemical sequencing reactions were performed as in Materials and Methods. The junction positions between the top and the bottom strands of the 25C/15 substrate and the BfiI recognition sequence are indicated.