Figure 4.

NPR1 Harbors an Autonomous Transactivation Domain in the Last 80 Residues.

(A) Histograms illustrating the transcriptional activity of the NPR1 BTB/POZ domain, the deletion mutants of the BTB/POZ, and the Ala substitution mutant tethered to DNA through Gal4 DB. The deletion and the Ala substitution mutants were created starting with the NPR1 BTB/POZ domain. The BTB/POZ region represents the first 190 amino acids of NPR1.

(B) Scheme of NPR1 and the deletions analyzed in (C). Numbers preceded by Δ indicate the starting amino acid for that particular deletion mutant. NLS indicates the nuclear localization signal. Ankyrin represents the region containing the ankyrin repeats as defined by Pfam and SMART. Diagram is drawn to scale.

(C) Histograms illustrating the transcriptional activity of the NPR1 deletion mutants described for (B) tethered to DNA through Gal4 DB.

For (A) and (C), conditions were identical to those described for Figure 1. Data are reported as relative luciferase units. Values are from 25 samples and represent averages ± sd. Every bar represents five bombardments repeated five times (n = 25).