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The Ankyrin Domain of XB3 Is Sufficient for in Vitro Interactions with XA21K.
35S-labeled XA21K was incubated with either resin-bound MBP, MBP-XB3, or MBP-XB3ankyrin (the ankyrin domain of XB3). After extensive washing, the resin-bound proteins were resolved by 8% SDS-PAGE. Both the Coomassie blue–stained gel (left) and its autoradiogram (right) are shown. XA21K visualized by autoradiogram is indicated. The experiment was repeated three times with similar results.
