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Cloned Arabidopsis THA1 and THA2 Thr Aldolases Relieve the Auxotrophy of Yeast Strain YM13 on Plates without Gly.
The top half of each plate is the W3031B wild-type GLY1;SHM1;SHM2 haploid strain; the bottom half is the isogenic Gly auxotrophic strain YM13 gly1;shm1;shm2. At the right, promoter expression is repressed with 10 μg/mL tetracycline. 1, THA1 clone; 2, THA2 clone; 3, pCM185 vector control; 4, no plasmid control.
