Figure 1.

mag2 Mutants Abnormally Accumulate the Precursors of Storage Proteins, 12S Globulins and 2S Albumins, in Seeds.

(A) Immunoblot of dry seeds of the wild type and mag2 mutants with anti-12S globulin antibodies and anti-2S albumin antibodies. The mag2 seeds accumulated large amounts of the precursors, pro12S globulin (p12S) and pro2S albumin (p2S), whereas wild-type seeds did not.

(B) Protein profiles of dry seeds (five grains) of the wild-type and mag2 mutants. 12S-α and 12S-β, 12S globulin subunits; 2S-L and 2S-S, 2S albumin subunits.

(C) The N-terminal sequences of the 17- and 54-kD proteins that were accumulated in mag2 mutants were determined. These sequences corresponded to the proprotein precursors of 12S globulin (12S1) and 2S albumin (2S3), respectively.