Figure 5.

The Novel Structures of the mag2 Mutant Accumulate ER Chaperones in the Matrix Region.

(A) Immunogold analysis of mag2-3 seeds showing the localization of BiP and PDI in the matrix region of the novel structures. Bars = 0.5 μm.

(B) Immunoelectron micrograph of mag2-2 seeds with anti-12S, showing the structure surrounded with ribosomes. Bar = 0.5 μm.

(C) Developmental changes in the levels of ER chaperones, including BiP, ER-localized Hsp90 (Hsp90/ER), and PDI, in maturing seeds of the wild type and mag2-1. Siliques at different developmental stages were alternately harvested starting from the top (just under the buds) of a wild-type plant.