Effects of BPBT Gene Knockout on the Histological Structure of Petunia Stems and Roots.
Cross sections of the middle part of the first-order internodes of 3-week-old nonflowering plants, stained with Mäule reagent, showed increased secondary growth and a proliferation of vascular tissue, particularly the xylem in BPBT RNAi-10 transgenic plants (B) in comparison with the control (A). Root cross sections of control (C) and BPBT RNAi-10 (D) transgenic plants showed a doubling of central xylem (x) and xylem pole (xp) cells and a loss of uniformity in cortical cells (c) of knockout plants.