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. 1999 Aug 31;96(18):10170–10175. doi: 10.1073/pnas.96.18.10170

Figure 5.

Figure 5

Expression and nuclear localization of S. cerevisiae Bir1-domain fragments fused to GFP. (A) Three GFP fusion constructs were designed to carry the BIR domains (shaded), the putative nuclear localization sequence (white), and/or the conserved C-terminal domain (shaded). The yeast strain YAU11 (Δbir1/Δbir1) was transformed with plasmids encoding Bir1(1–247)-GFP (B), Bir1(1–474)-GFP (C), or Bir1(249–954)-GFP (D). Expression of GFP fusions in mid-log-phase cells was analyzed by fluorescence microscopy.