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Interaction of SipA with T-Plastin and actin in the yeast two-hybrid system. Plasmids expressing different truncated forms of SipA (stippled box) fused to the Gal4-binding domain (hatched box) as indicated were transformed into a yeast indicator strain expressing a fusion between the Gal4 activation domain and the C-terminal 300 aa of human T-plastin (pSB1014) or the entire yeast actin (pAAB421). The β-galactosidase activity is expressed in Miller units.
