Figure 2.

Interaction of SipA with plastin in vivo and in vitro. (A) Interaction of SipA with plastin examined with a GST pull-down assay. The presence of actin and plastin bound to the GST-SipA beads (Pellet), unbound in HeLa cell extracts (Post), or before the pull-down (Pre) was detected by immunoblot with an anti-actin (Upper) or anti-plastin (Lower) antibody. (B) SipA-plastin complex formation in vivo. HeLa cells were infected with either wild-type S. typhimurium or the sipA mutant strain, and the interaction in cell lysates between SipA and actin, plastin, α-actinin, or vinculin was investigated by coimmunoprecipitation analysis. IP, immunoprecipitate.