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The decay in Stat1 DNA binding in cytoplasmic and nuclear extracts during an IFN-γ time course. (A and B) EMSA analysis with an M67 probe. Cytoplasmic (A) and nuclear (B) extracts were prepared from Bud-8 normal human fibroblasts treated with IFN-γ for the indicated times. (C) Plot of the PhosphorImager analysis of Stat1 DNA binding complexes at various times after IFN-γ treatment.
