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The decay in Stat1 DNA binding in cytoplasmic and nuclear extracts upon staurosporine addition. (A and B) EMSA analysis with an M67 probe. Cytoplasmic (A) and nuclear (B) extracts were prepared from Bud-8 normal human fibroblasts treated with IFN-γ for 30 min followed by staurosporine (500 nM) for the indicated times. (C) Plot of the PhosphorImager analysis of Stat1 DNA binding complexes at various times after IFN-γ plus staurosporine treatment.
