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The effect of vanadate on Stat1 DNA binding in cytoplasmic and nuclear extracts. (A and B) EMSA analysis with an M67 probe. Cytoplasmic (A) and nuclear (B) extracts were prepared from Bud-8 normal human fibroblasts treated with IFN-γ plus vanadate for the indicated times. (C) Plot of the PhosphorImager analysis of Stat1 DNA binding complexes at various times after IFN-γ plus vanadate treatment.
