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Bacterially expressed Hap46. GST fusion proteins of wild-type and mutant Hap46 were expressed in E. coli, purified by affinity chromatography, released by thrombin cleavage, and analyzed by SDS/PAGE. Full-length Hap46 (lane 1), Hap46ΔN10 (lane 2), Hap46 K(2-4)→A (lane 3), Hap46 R(6-8)→A (lane 4), and Hap46ΔC47 (lane 5) were used. Staining was performed with Coomassie blue R250.
