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Temperature effect on the interaction of Hap46 with hsc70. The GST-Hap46 fusion protein (5 μg) was incubated with hsc70 (1 μg) and GSH-Sepharose for 1 h at 37°C followed by 1 h at either 37° (lane 1) or 42°C (lane 2). Bound protein was analyzed by immunoblotting with hsc70-specific antibody N27F3-4. Quantitation was performed by densitometric scanning and showed that, in the control experiment (lane 1), hsc70 had been retained quantitatively on the matrix.
