Figure 6.
Hap46 stimulates in vitro transcription. Transcription assays were done with [32P]GTP and nuclear extracts from HeLa cells. Labeled RNA was analyzed by gel electrophoresis and autoradiography. Transcription reactions occurred in the absence (lane 1) or presence of full-length Hap46 (lanes 2 and 3) or mutant Hap46ΔN10 (lane 4) with a CMV promoter carrying DNA template. In the experiment shown in lane 3, Hap46 was used in combination with hsc70. The position of a 300-nt RNA is indicated.