Figure 2.

NDC1 haploinsufficiency survival mechanisms. Schematic of the mechanisms by which diploid strains that are heterozygous for the ndc1 null allele survive in the absence of a plasmid-borne copy of NDC1. The starting yeast strain (Haploinsufficient Parent Yeast Strain) has both copies of chromosome XIII, where NDC1 resides, marked with specific genes corresponding to the ndc1 null-containing chromosome (HIS3) or the NDC1-containing chromosome (TRP1 and KANMX). This yeast strain also contains a plasmid-borne copy of NDC1 with the URA3 and ADE3 markers.