Transcription by the conserved domain in TBX3 and by TBX2. Each test plasmid (0. 1 μg) was transfected into 293T cells. Column 1, 293T cells were cotransfected with the reporter plasmid and carrier DNA, and the luciferase activity is 100 ± 4.8; column 2, cotransfection with Gal4–ET, column 3; cotransfection with Gal4–hTBX2; column 4, cotransfection with Gal4–hTBX3(524–674) carrying a point mutation (Ser-542 → Leu-542) outside the predicted repressor domain; column 5, cotransfection with Gal4–hTBX3(524–674).