Figure 5.

Effects of DALP and Hic-5 expression on C₂C₁₂ myoblasts. C₂C₁₂ myoblasts were grown in growth (A, C, E, and G) or differentiation (B, D, F, and H) medium and then stained with a mAb against myosin heavy chain (brown). (A and B) Wild-type cells. (C and D) C₂C₁₂ line constitutively expressing DALP. (E and F) C₂C₁₂ line constitutively expressing exogenous Hic-5. No myotubes were formed from DALP- or Hic-5-expressing cells. (F and G) Hic-5-expressing cells infected with a MyoD-expressing retroviral construct. Ectopic MyoD expression can abrogate the effects of Hic-5 on myotube formation. Arrowheads point to myosin heavy chain-positive mononucleated myoblasts in growth medium.