Figure 7.
Effects of DALP and Hic-5 expression on myotube formation in C2C12 cells. Myoblasts were transiently transfected with expression vectors that coexpressed DALP and β-galactosidase (A) or Flag-tagged Hic-5 (B). After 48 hr, cultures were transferred to differentiation medium. After an additional 3 days, cells were processed for β-galactosidase (A) or Flag (B) immunohistochemistry. In separate experiments, BrdUrd-labeled Hic-5-expressing C2C12 cells were mixed with wild-type cells and then placed in differentiation medium. Arrows, nuclei from wild-type cells; arrowheads, BrdUrd-positive nuclei. The micrograph is overexposed to enhance the visualization of the control nuclei.