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. 1999 Aug 31;96(18):10284–10289. doi: 10.1073/pnas.96.18.10284

Figure 4.

Figure 4

Complementation test. (A) Transgenic plants of d1 mutant (HO541). (1) Left, transgenic plant with cosmid vector; right, transgenic plant with cosmid clone (C6) containing D1 genomic region. (2) Left, seeds of transgenic plant with cosmid vector; right, seeds of transgenic plant with cosmid clone (C6). (B) Detection of integrated D1 gene in transgenic plants. Lanes: 1, marker (ϕX174/HaeIII); 2, Nipponbare (normal); 3, HO541 (d1 mutant); 4, cosmid clone (C6); 5–8, transgenic plants with cosmid vector in HO541 (phenotypes were all dwarf); 9–12, transgenic plants with cosmid clone C6 (phenotypes were all normal); 13, marker (ϕX174/HaeIII).