Figure 4.

Requirement of DAS for complementation of rocG mutations. Plasmids pBB921 or pBB967 (Fig. 2B) were integrated in the chromosome of two different rocG deletion mutants (strains BB1284 and BB1541) either at the rocG locus or at the ectopic amyE locus. The full-length rocG allele and the DAS are presented as filled boxes. The vector part of the plasmids (10.5 kb) and the DAS (61 bp) are not to scale. The locations of the rocG and rocA promoter regions are indicated by right-angle arrows. Growth phenotype was scored as ability to use ornithine or proline as sole carbon and nitrogen source. GlutDH activity was determined in succinate-glutamate-ornithine medium.