Table 2.

RocG-GlutDH activity

Strain (genotype)	Carbon and nitrogen sources	RocG activity
BB1268 (wild-type)	Succinate + glutamate	<40
BB1268 (wild-type)	Succinate + glutamate + ornithine	649
BB1552 (sigL::aphA3)	Succinate + glutamate + ornithine	<40
BB1553 (rocRΔ3::aphA3)	Succinate + glutamate + ornithine	<40
BB1542 [rocR(T120I)]	Succinate + glutamate	763
BB1542 [rocR(T120I)]	Succinate + glutamate + ornithine	829
BB1680 (ahrC::aphA3)	Succinate + glutamate + ornithine	<40

The null mutations in sigL, rocR, rocD, and ahrC and the constitutive rocR(T120I) mutation were as described (10, 13, 14). All strains carried the ΔgudB::tet mutation to avoid any contribution from GudB-GlutDH(17).