Table 3.

RocG-GlutDH activity in the absence of the DAS

Strain (genotype)	RocG activity
BB1664 (ΔDAS/UAS)	<40
BB1667 [ΔDAS/UAS amyE::rocG (DAS/UAS+)]	683
BB1668 [ΔDAS/UAS amyE::rocG (DAS/UAS−)]	<40
BB1665 (ΔDAS/UAS rocD::aphA3::spc)	65
BB1666 (ΔDAS/UAS rocD::aphA3::spc rocRΔ3::aphA3)	<40

Strain BB1664 containing the 106-bp DAS/UAS deletion and its derivatives were grown in succinate-glutamate-ornithine medium. The null mutations in rocD and rocR were described, respectively, in Material and Methods and in ref. 13. All strains carried the ΔgudB::tet mutation to avoid any contribution from GudB-GlutDH (17).