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Expression of a rocG-lacZ fusion containing the DAS/UAS in the upstream position
| Strain (genotype) | β-galactosidase activity in different
media
|
|
|---|---|---|
| Succinate Glutamate | Succinate Glutamate Ornithine | |
| BB1501 (wild-type) | 2.1 | 264.9 |
| BB1503 (sigL::aphA3) | 1.2 | 1.2 |
| BB1502 (rocRΔ3::aphA3) | 2.0 | 1.4 |
| BB1522 [rocR(T120I)] | 122.6 | 225.3 |
Strain BB1501 and its derivatives carry a rocG-lacZ fusion [as in pBB986, (Fig. 5)] at the amyE locus.
