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. 1999 Aug 31;96(18):10308–10313. doi: 10.1073/pnas.96.18.10308

Figure 1.

Figure 1

Expression of α1AT, CBG, HNF-1α, and HNF-4 mRNAs in parental cells, microcell hybrids, and transfectants. Cytoplasmic RNAs (8 μg/lane) from a rat hepatoma hybrid containing human chromosome 14 [F(14n)2], Fg-14 rat hepatoma cells, the H11 rat hepatoma variant, H11 variants containing human chromosome 14 [H11(14n)D and H11(14n)E], rescued variants expressing HNF-4 [(EH4)6B, (EH4)6X] or HNF-1α [(EH1)3J], and a rat fibroblast microcell hybrid containing human chromosome 14 [R(14n)6] were analyzed by Northern blot hybridization. The α1AT and CBG probes used were human cDNAs that did not cross-hybridize to rat mRNAs under the conditions used. Loading controls were ethidium bromide (EtBr)-stained gels and hybridization with a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA probe (54).