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. 1999 Aug 31;96(18):10308–10313. doi: 10.1073/pnas.96.18.10308

Figure 2.

Figure 2

DHS mapping and generalized DNase I-sensitivity assays in microcell hybrids. Nuclei from human chromosome 14-containing rat hepatoma [F(14n)2], variant [H11(14n)D, H11(14n)E], and fibroblast [R(14n)6] cells were digested with DNase I, and DNA was purified, digested with BamHI (A), BglII (B), or XbaI (C) and analyzed by Southern hybridization. The probes for the DHS mapping experiments were a 0.7-kb EcoRI–HindIII fragment from map position ≈-21.5 kb (A) and a ≈0.95-kb BglII–AvaI fragment from map position ≈+36 kb (B). The probes for the generalized DNase I-sensitivity assays (C) were a 1.4-kb XhoI–HindIII genomic fragment containing exon III of the α1AT gene from cosmid αATc1 (52) and a ≈1.0-kb XbaI–PstI genomic fragment containing exon II from cathepsin G (55). Both probes hybridized with DNA fragments of ≈2.4 kb in XbaI digests of genomic DNA.