Figure 4.

HNF-1α or HNF-4 induces long-range chromatin reorganization of the human α1AT-ATR-CBG locus in H11 variant cells. Nuclei from an H11 variant containing human chromosome 14 [H11(14n)E] and rescued variant transfectants expressing HNF-4 [(EH4)6B, (EH4)6X] or HNF-1α [(EH1)3J] were treated with DNase I, and isolated DNA was cleaved with HindIII (A and B) or EcoRI (C). The probes were a 0.9-kb SpeI–HindIII fragment (+2.7 to +3.6 kb) (A); a 0.6-kb BglII fragment (+67.7 to +68.3 kb) (B); and a 2.2-kb HindIII fragment (−4.9 to −2.7 kb) (C). Solid arrows indicate DHSs that were induced in the rescued variants. Open arrows in A indicate a constitutive DHS that was found in all cell lines.