Figure 3.

Gel filtration pattern of the partially purified HP from an HP2-1 individual (A) and from the case I variant (B). The purified HP molecules after reduction with 2-mercaptoethanol and carboxymethylation with iodoacetoamide were separated on a TSK 3000SW column. The polypeptides were monitored by 280-nm UV absorbance. The polypeptide recovered from the P3 fraction of the case I HP variant was regarded to be HPα1S. The protein level of the plasma HP of the variant was in the normal range, but the molar ratio of the α1S polypeptide to the αS2 polypeptide was less than 1:3.