Skip to main content
. 1999 Aug 31;96(18):10314–10319. doi: 10.1073/pnas.96.18.10314

Figure 4.

Figure 4

Southern blot analysis of HP genotypes of B cell colonies. B cells from the case I variant were transformed by EBV and cloned in soft agarose. Genomic DNAs were digested with HindIII, and the Southern blots were hybridized to the hapto 6 probe (1.4-kb PstI fragment of HP cDNA). The sample in lane 11 was an HP*2/HP*2 control. The 4.9-kb band corresponded to the HP2, and the 3.4-kb band to the HP related gene (HPR) band (5). The 3.2-kb bands (shown with ■) in lanes 1, 3, and 4 were putative HP1 bands. Nucleotide sequencing of the 3.2-kb bands confirmed that they are identical with the sequence of the HP1S gene of Maeda et al. (5). A total of 41 B cell colonies were obtained for genotyping; 33 colonies were HP*2/HP*2 homozygotes, and eight were HP*2/HP*1 heterozygotes. Ten of these are represented.