Co-IP of transiently expressed FANCA and FANCG proteins. Protein from 293-EBNA cell extracts, mock transfected (lanes 1), or expressing FANCA-FLAG (lanes 2), HA-FANCG (lanes 3), or FANCA-FLAG + HA-FANCG (lanes 4) was (A) immunoprecipitated with monoclonal FLAG antibody (M2) and immunoblotted with anti-FLAG (Upper) or anti-HA (Lower) or (B) immunoprecipitated with monoclonal HA antibody (12CA5) and immunoblotted with anti-HA (Upper) or anti-FLAG (Lower). Large arrowheads indicate FANCA-FLAG or HA-FANCG, small arrowheads indicate Ig heavy chains.