Figure 4.
FANCA/FANCG complex in FA-G cells. (A) Cell extracts (1 mg per IP) from control cell line HSC93 (lane 1), FA-G cell lines EUFA143 (lane 2), EUFA349 (lane 3), EUFA316 (lane 4), and FA-G cell line EUFA316 corrected with HA-FANCG in pMEP4 (lane 5) were immunoprecipitated with anti-FANCG480–622 and immunoblotted with rabbit FANCA antiserum. (B) Similar lysates as in A were immunoprecipitated with anti-FANCA1–271 and immunoblotted with rabbit anti-FANCA (Upper) or with anti-HA (Lower). Arrowheads indicate FANCA in A and B (Upper) and HA-FANCG in B (Lower). Mutations in FA-G lines are: EUFA143, homozygous E105X; EUFA316, frameshift at amino acid 395 and E105X; EUFA349, frameshift at amino acid 479 and E105X (8).