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. 1999 Aug 31;96(18):10320–10325. doi: 10.1073/pnas.96.18.10320

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Copyright © 1999, The National Academy of Sciences

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FANCA/FANCG complex in FA-A and FA-C cells. Immunoblotting of anti-FANCG_480–622 (Upper) or anti-FANCA_1–271 (Lower) precipitated proteins with rabbit anti-FANCA. Cell extracts were prepared from HSC93 control cells (lanes 1), (A) HSC72 (lane 2), corrected HSC72(FANCA-FLAG) (lane 3), HSC536 (lane 4), and corrected HSC536(FLAG-FANCC) (lane 5). (B) Homozygously mutated FA-A cell lines; BD32 (H1110P) (lane 2), EUFA689 (1263delF) (lane 3), and EUFA268 (Q772X) (lane 4). (C) Homozygously mutated FA-C cell lines; EUFA166 (322delG) and GM4510 (IVS4), (lanes 2 and 3, respectively). In all precipitations 1 mg of protein was used.