Figure 4.

Filter lift hybridizations, restriction fragment length polymorphism, and sequence analysis of DNA isolated from liver. (A) Hybridization patterns of duplicate filter lifts of the cloned PCR products from liver DNA of Gunn rats 6 months postinjection with vehicle (Upper) or UGT1A1 ONs (Lower). (B) PCR amplicons were subjected to BstNI restriction enzyme digestion and analyzed by agarose gel electrophoresis and ethidium bromide staining (Top). Direct DNA sequencing of the PCR-amplified UGT1A1 gene surrounding the targeted G insertion site at position 1206 (arrow) is shown for wild-type (G, top sequence), vehicle (A, middle), and UGT1A1-treated Gunn rats (A and G, bottom). The size of the DNA standards is indicated at top left.