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Immunolocalization of p53 in cells with wild-type or mutant p53. MCF-7 breast carcinoma cells (wt p53) and HT-29 colon carcinoma cells (mutant p53) were treated with the DNA-damaging agent mitomycin C (10 μg/ml) for 4 hours followed by a chase in drug-free media for an additional 20 hours. The cellular localization of p53 was determined by immunohistochemistry with the PAb 1801 monoclonal anti-p53 antibody followed by FITC-conjugated anti-mouse secondary antibodies.
