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Western blot analysis of nuclear extracts from mitomycin C-treated cells. MCF-7 breast carcinoma cells (wt p53) and HT-29 colon carcinoma cells (mutant p53) were treated with the DNA-damaging agent mitomycin C (10 μg/ml) for 4 hours followed by a chase in drug-free media for an additional 20 hours. Nuclear extracts from MCF-7 cells (A and C) and HT-29 cells (B) were subjected to Western blot analysis with antibodies directed toward p53 (A and B) or topoisomerase I (C).
