Figure 2.

OLIGO cells coexpress NG2 and PDGFα-R but not GFAP or LCA. (a–c) Sections from an OLIGO double-labeled with antibodies to PDGFα-R (a, green) and NG2 (b, red). Merged images demonstrate colocalization of NG2 and PDGFα-R (c, yellow). (d–f) Double-labeling with antibodies to PDGFα-R (d, green) and GFAP (e, red). No colocalization is observed in the merged image (f). (g–i) Double-labeling with antibodies to NG2 (g, red) and LCA (h, green). No colocalization is observed in the merged image (i). Tumor 3. [Bar = 40 μm (a–c), 30 μm (d–f), and 20 μm (g–i).]