Table 1.
NG2 and PDGFα-R immunostain and Western blot analysis of 16 brain tumors
| Tumor no. | Diagnosis | NG2 immunostain
|
PDGFα-R
|
||
|---|---|---|---|---|---|
| TC* | BV† | TC* | Western blot‡ | ||
| 44 | OLIGO | +++ | + | − | ++ |
| 2 | OLIGO | +++ | − | +++ | + |
| 39 | OLIGO | +++ | + | +++ | + |
| 30 | OLIGO | +++ | + | +++ | ++ |
| 3 | AOLIGO | +++ | + | +++ | + |
| 48 | AOLIGO | +++ | − | ++ | ++ |
| 10§ | AOLIGO | + | + | − | ++ |
| 40 | PA | + | − | ++ | ++ |
| 58 | PA | +++ | − | +++ | ND |
| 17 | PA | ++ | + | ++ | ++ |
| 47 | GBM | − | + | − | + |
| 54 | GBM | ++ | + | ++ | + |
| 45 | GBM | − | + | − | ++ |
| 57 | GBM | − | + | − | + |
| 4 | GBM | − | − | − | + |
| 24 | A | − | + | − | ++ |
ND, not done.
*
NG2 or PDGFα-R immunoreactivity in tumor cells (TC). −, No detectable immunoreactive TC; +, <5% immunoreactive TC; ++, 5–50% immunoreactive TC; +++, >50% immunoreactive TC.
†
NG2 immunoreactivity in blood vessels. Immunoreactive vessels scored as present (+) or absent (−).
‡
PDGFα-R band (180 kD). +, detectable band present; ++, intense band present.
§
This specimen consisted of mildly hypercellular gray matter infiltrated by immunoreactive TC.