Abstract
Sequence analysis showed that the cyd operon is immediately upstream of the tol-pal region. Northern (RNA) blot analysis demonstrated that the transcript for the cyd operon terminates just before the promoter for transcription of the tol genes. The cyd transcript contains cydA cydB followed by two open reading frames: orfC, encoding a 37-residue peptide, and orfD, encoding a 97-residue peptide. Both OrfC and OrfD are synthesized in minicells.
Full Text
The Full Text of this article is available as a PDF (543.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bradley D. E., Whelan J. Escherichia coli tolQ mutants are resistant to filamentous bacteriophages that adsorb to the tips, not the shafts, of conjugative pili. J Gen Microbiol. 1989 Jul;135(7):1857–1863. doi: 10.1099/00221287-135-7-1857. [DOI] [PubMed] [Google Scholar]
- Cotter P. A., Gunsalus R. P. Contribution of the fnr and arcA gene products in coordinate regulation of cytochrome o and d oxidase (cyoABCDE and cydAB) genes in Escherichia coli. FEMS Microbiol Lett. 1992 Feb 1;70(1):31–36. doi: 10.1016/0378-1097(92)90558-6. [DOI] [PubMed] [Google Scholar]
- Dassa J., Fsihi H., Marck C., Dion M., Kieffer-Bontemps M., Boquet P. L. A new oxygen-regulated operon in Escherichia coli comprises the genes for a putative third cytochrome oxidase and for pH 2.5 acid phosphatase (appA) Mol Gen Genet. 1991 Oct;229(3):341–352. doi: 10.1007/BF00267454. [DOI] [PubMed] [Google Scholar]
- Davies J. K., Reeves P. Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group A. J Bacteriol. 1975 Jul;123(1):102–117. doi: 10.1128/jb.123.1.102-117.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fang H., Gennis R. B. Identification of the transcriptional start site of the cyd operon from Escherichia coli. FEMS Microbiol Lett. 1993 Apr 1;108(2):237–242. doi: 10.1111/j.1574-6968.1993.tb06105.x. [DOI] [PubMed] [Google Scholar]
- Green G. N., Fang H., Lin R. J., Newton G., Mather M., Georgiou C. D., Gennis R. B. The nucleotide sequence of the cyd locus encoding the two subunits of the cytochrome d terminal oxidase complex of Escherichia coli. J Biol Chem. 1988 Sep 15;263(26):13138–13143. [PubMed] [Google Scholar]
- Horabin J. I., Webster R. E. Morphogenesis of f1 filamentous bacteriophage. Increased expression of gene I inhibits bacterial growth. J Mol Biol. 1986 Apr 5;188(3):403–413. doi: 10.1016/0022-2836(86)90164-6. [DOI] [PubMed] [Google Scholar]
- Larsen J. E., Gerdes K., Light J., Molin S. Low-copy-number plasmid-cloning vectors amplifiable by derepression of an inserted foreign promoter. Gene. 1984 Apr;28(1):45–54. doi: 10.1016/0378-1119(84)90086-6. [DOI] [PubMed] [Google Scholar]
- Lazzaroni J. C., Portalier R. C. Genetic and biochemical characterization of periplasmic-leaky mutants of Escherichia coli K-12. J Bacteriol. 1981 Mar;145(3):1351–1358. doi: 10.1128/jb.145.3.1351-1358.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lazzaroni J. C., Portalier R. The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein (PAL). Mol Microbiol. 1992 Mar;6(6):735–742. doi: 10.1111/j.1365-2958.1992.tb01523.x. [DOI] [PubMed] [Google Scholar]
- Levengood S. K., Webster R. E. Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli. J Bacteriol. 1989 Dec;171(12):6600–6609. doi: 10.1128/jb.171.12.6600-6609.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Russel M., Whirlow H., Sun T. P., Webster R. E. Low-frequency infection of F- bacteria by transducing particles of filamentous bacteriophages. J Bacteriol. 1988 Nov;170(11):5312–5316. doi: 10.1128/jb.170.11.5312-5316.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sun T. P., Webster R. E. Nucleotide sequence of a gene cluster involved in entry of E colicins and single-stranded DNA of infecting filamentous bacteriophages into Escherichia coli. J Bacteriol. 1987 Jun;169(6):2667–2674. doi: 10.1128/jb.169.6.2667-2674.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sun T. P., Webster R. E. fii, a bacterial locus required for filamentous phage infection and its relation to colicin-tolerant tolA and tolB. J Bacteriol. 1986 Jan;165(1):107–115. doi: 10.1128/jb.165.1.107-115.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vianney A., Muller M. M., Clavel T., Lazzaroni J. C., Portalier R., Webster R. E. Characterization of the tol-pal region of Escherichia coli K-12: translational control of tolR expression by TolQ and identification of a new open reading frame downstream of pal encoding a periplasmic protein. J Bacteriol. 1996 Jul;178(14):4031–4038. doi: 10.1128/jb.178.14.4031-4038.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wall D., Delaney J. M., Fayet O., Lipinska B., Yamamoto T., Georgopoulos C. arc-dependent thermal regulation and extragenic suppression of the Escherichia coli cytochrome d operon. J Bacteriol. 1992 Oct;174(20):6554–6562. doi: 10.1128/jb.174.20.6554-6562.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Webster R. E. The tol gene products and the import of macromolecules into Escherichia coli. Mol Microbiol. 1991 May;5(5):1005–1011. doi: 10.1111/j.1365-2958.1991.tb01873.x. [DOI] [PubMed] [Google Scholar]
- Wray L. V., Jr, Jorgensen R. A., Reznikoff W. S. Identification of the tetracycline resistance promoter and repressor in transposon Tn10. J Bacteriol. 1981 Aug;147(2):297–304. doi: 10.1128/jb.147.2.297-304.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zuker M. On finding all suboptimal foldings of an RNA molecule. Science. 1989 Apr 7;244(4900):48–52. doi: 10.1126/science.2468181. [DOI] [PubMed] [Google Scholar]