Table 1.
Comparison of anti-cell CD4-reactive antibodies and anti-rsCD4 antibodies for binding characteristics and ability to neutralize a primary isolate of HIV
| Antibody | Binding to rsCD4, A492 | Fluorescent staining of HPB-ALL
|
Antibody concentration at 50% neutralization | |
|---|---|---|---|---|
| Percent | Intensity* | |||
| B4 | 1.424 | >90% | 3+ | 0.21 μg/ml |
| M2 | 0.871 | >90% | 3+ | 0.38 μg/ml |
| D5 | 1.930 | >90% | 1+ | >10 μg/ml |
| E2 | 2.020 | >10% | 1+ | >10 μg/ml |
| I26 | 0.793 | 0 | 0 | >10 μg/ml |
| E6 | 2.007 | >90% | 2+ | 59 μg/ml |
| H5 | 1.984 | >90% | 1+ | 45.5 μg/ml |
| E31 | 0.936 | >90% | 1+ | >100 μg/ml |
| J33 | 2.059 | >90% | 1+ | >100 μg/ml |
| GP anti-rsCD4 | >105† | >90% | 3+ | <1:10 dilution |
B4, M2, D5, and I26 are anti-HPB-ALL. E6, H5, J33 and “GP anti-rsCD4” are anti-rsCD4.
*
0, faint cell image, no bright spots; 1+, small bright spots, numerous; 2+, double sized bright spots; 3+, most of cell surface brightly fluorescent.
†
Serum dilution endpoint at which A492 > 0.154.