Figure 1.
Generation of PLC β3-null mice. (A) Schematic representation of a PLC molecule. The X and Y boxes comprise the catalytic domain. The solid box represents the exon manipulated in the gene-targeting construct. (B) Gene-targeting construct and the homologous recombination process. (C) Western analysis of DRG neurons isolated from the mice (+/+, wild type; +/−, heterozygous; −/−, homozygous) with a PLC β3-specific antibody.