Figure 5.

Epoxomicin inhibits inflammation in vivo. (A) CS to picrylchloride. BALB/c mice were immunized with picrylchloride by topical application to shaved chest and abdomen. One group of immunized mice (n = 4) was treated with epoxomicin daily from the time of immunization until the time of ear challenge, whereas the control immunized group (n = 4) was treated with vehicle. On day 6 postimmunization, all mice were skin-challenged by painting both ears with 0.8% picrylchloride. Ear swelling responses were measured before (0 hr) and 24 hr after ear challenge. (B) Irritant sensitivity (IS) response to picrylchloride. Nonimmunized BALB/c mice were injected with epoxomicin (n = 4) or vehicle (n = 4) before ear challenge with 0.8% picrylchloride, and ear swelling measurements were made 0 and 24 hr post-ear challenge. Results in CS and IS assays are expressed as 24-hr measurement minus 0-hr measurement.