Figure 5.

B. subtilis IRE-like structures. (A) Sequences of the consensus eukaryotic IRE and putative IREs located 3′ of the qoxD coding sequence and in-between the feuA and feuB genes of B. subtilis. (B) The sequences shown in A were cloned and transcribed in vitro and were tested for binding to purified B. subtilis aconitase preparations. Similar binding reactions were performed by using a purified preparation of rabbit IRP1. Dipyridyl, to a final concentration of 0.5 mM, was added to the binding reactions as indicated.