Table 2.
Classification of 71 selected genes encoding putative secretory products as determined by RT-PCR expression analysis on female salivary glands, whole male mosquitoes, and carcass of female mosquitoes
| Protein name or type | Comments | SG | ENR | SG, M | Ubiq | # |
| Secreted carrier-like proteins including D7 family | ||||||
| gi|61742027 | D7s1 | X | ||||
| gi|94468624 | D7s2 allele | X | ||||
| gi|18568330 | D7s3 | X | # | |||
| gi|16225992 | D7l1 | X | ||||
| gi|118216 | D7l2 | X | ||||
| gi|94468424 | D7s4 | X | ||||
| Secreted protease inhibitors | ||||||
| Serpins | ||||||
| gi|94468342 | Similar to FXa-directed anticlotting | X | # | |||
| gi|94469320 | Salivary serpin | X | ||||
| gi|18568304 | Serpin | X | # | |||
| Other protease inhibitors | ||||||
| gi|94468720 | Kazal-containing peptide | X | ||||
| gi|94468612 | Cystatin | X | ||||
| Enzymes | ||||||
| Nucleotidases | ||||||
| gi|94469274 | Second apyrase or 5' nucleotidase | X | ||||
| gi|21654712 | Salivary purine nucleosidase | X | ||||
| gi|1703351 | Salivary apyrase | X | ||||
| gi|18568326 | Putative adenosine deaminase | X | ||||
| Serine proteases | ||||||
| gi|94468658 | Serine protease | X | # | |||
| gi| 94468410 | Salivary chymotrypsin-like enzyme | X | ||||
| gi|18568306 | Putative serine protease | X | ||||
| gi|94468372 | Trypsin like | X | ||||
| Immunity-related proteins | ||||||
| Lectins | ||||||
| gi|94468370 | Putative salivary C-type lectin | X | ||||
| gi|18568318 | Putative C-type lectin | X | # | |||
| gi|94468698 | C type lectin | X | ||||
| gi|94468694 | C-type lectin | X | ||||
| Angiopoietin | ||||||
| gi|18568298 | Angiopoietin-like protein | X | # | |||
| gi|94468352 | Angiopoietin-like protein splice variant | X | ||||
| Antimicrobial polypeptides | ||||||
| gi|48256697 | Defensin A1 | X | ||||
| gi|18568310 | Gambicin | X | ||||
| gi|18568288 | Putative lysozyme | X | ||||
| gi|94468690 | Putative salivary peptide with HHH domain | X | ||||
| Other | ||||||
| gi|94468654 | i23M allele | X | ||||
| gi|18568294 | Gram-negative binding protein | X | ||||
| gi|94468610 | Peptidoglycan recognition protein | X | ||||
| Unknown function, secreted, and ubiquitous | ||||||
| Antigen 5 protein family | ||||||
| gi|18568284 | Antigen 5 member | X | ||||
| gi|18568278 | Putative secreted protein | X | ||||
| gi|18568308 | Antigen 5 family member | X | ||||
| Other secreted proteins found in non-bloodsucking insects | ||||||
| gi|94468620 | PAN/APPLE-like domains | X | ||||
| gi|94468538 | Cysteine-rich venom-like protein | X | # | |||
| Unknown function, found in hematophagous Diptera salivary transcriptomes | ||||||
| 56.5 kDa | ||||||
| gi|18568292 | Putative 56.5 kDa secreted protein | X | ||||
| 41-kDa mosquito family | ||||||
| gi|94468350 | Putative 41-kDa salivary secreted protein | X | # | |||
| 30-kDa/GE-rich family | ||||||
| gi|94468546 | 30-kDa salivary gland allergen variant 2 | X | # | |||
| gi|18568322 | Putative 30-kDa allergen-like protein | X | # | |||
| 29-kDa family | ||||||
| gi|94468416 | Putative salivary protein | X | ||||
| Other salivary proteins found in hematophagous Diptera | ||||||
| gi|94468392 | Putative secreted protein | X | ||||
| gi|94468374 | Similar to An. gambiæ gSG8 protein | X | ||||
| gi|61742023 | Putative 8.9-kDa secreted protein | X | ||||
| gi|94468386 | Putative 23.4-kDa salivary protein | X | ||||
| gi|94468986 | Putative secreted peptide | X | ||||
| gi|94468460 | Putative secreted peptide | X | ||||
| gi|94468614 | Unknown secreted | X | ||||
| gi|94468634 | Possible secreted protein | X | # | |||
| gi|94468408 | Putative secreted salivary peptide | X | ||||
| Aedes genus specific | ||||||
| 62kDa family – Single exon | ||||||
| gi|18568300 | Putative secreted protein | X | # | |||
| gi|18568302 | Putative secreted protein | X | # | |||
| 34-kDa gene family | ||||||
| gi|94468412 | Salivary peptide similar to 34-kDa protein | X | ||||
| gi|94468650 | Putative salivary peptide | X | ||||
| gi| 94468336 | Putative 34-kDa secreted protein family | X | ||||
| gi|18568296 | Putative 34-kDa secreted protein | X | ||||
| 30.5-kDa | ||||||
| gi|94468432 | Putative 30.5-kDa secreted protein | X | ||||
| gi|61742033 | Putative 30-kDa secreted protein | X | # | |||
| 9-kDa family | ||||||
| gi|94468426 | Salivary peptide with WWW domain | X | ||||
| gi|94468396 | Putative secreted peptide | X | ||||
| Other Aedes-specific polypeptides | ||||||
| gi|94468530 | Putative salivary secreted peptide | X | ||||
| gi|94468356 | Putative salivary peptide with basic tail | X | ||||
| gi|18568314 | Putative 18.6-kDa secreted protein | X | ||||
| gi|94468380 | Putative 18.6-kDa secreted protein – variant | X | ||||
| gi|18568282 | Putative 7.8-kDa secreted protein | X | # | |||
| gi|94468390 | Putative basic peptide 4.2K-1 | X | ||||
| gi|94468394 | Proline-rich peptide | X | ||||
| gi|94468568 | Putative salivary secreted peptide | X | ||||
| gi|42632615 | Putative 8.7-kDa secreted protein | X | # | |||
| gi|94468646 | Peptide similar to insulin-like growth factor | X | ||||
The four classes are SG (signal found exclusively in adult female salivary glands); ENR (enriched in adult female salivary glands); SG,M (found in adult female salivary glands and in males, but not in carcasses of adult females lacking salivary glands); and Ubiq (genes ubiquitously found in all tissues tested). Clones marked with # were analyzed three times, all others twice. Clones 94468370, 48256697, and 94468634 may undergo alternative splicing. Although clones 94468546 and 18568322 (30 kDa), 94468614, 18568300, and 18568302 (62 kDa) were assigned to SG class, some faint bands were seen in some samples from males. Clones 94468620, 94468350, 94468634, and 42632615 (primer pairs 23, 3, 29, and 60) underwent 35 cycles due to low amplification levels.