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. 2007 Feb 2;3(2):e1. doi: 10.1371/journal.pgen.0030001

Figure 4. Modification of Cell Proliferation Rate in 21A-Overexpressing HeLa Cells.

Figure 4

(A) Proliferation inhibition of HeLa cells after 48 h of 21A constructs transfection. Results emphasize the specificity of the Alu Jb-containing region as a source of proliferation inhibitory transcripts.

(B) Proliferation increase of HeLa cells after 48 h of pAnti-21A and si21A transfection. siEx-FABP, unrelated chicken-specific siRNA (negative control).

(C and D) Constructs structures. Anti-21A, the transcript region is inverted and the construct maintains 21A promoter as well as its termination site. si21A, siRNA 21A-specific.

(E and F) CENP-F protein expression level after 0, 24, and 48 h of constructs transfection. Full triangle, anti-CENP-F antibody; full circle, anti-tubulin antibody; striped columns, quantitative determination of CENP-F expression modulation as determined by Western blot analysis; full columns, quantitative determination of CENP-F mRNA expression modulation as determined real-time RT-PCR analysis.

(G and H) 21A RNA level in transfected samples indicating that the endogenous 21A expression is inhibited after 24 h of anti-21A/si21A transfection.